This is probably a naive question. Given an image stack with z-spacing that is not the same as x and y, what is the correct way to handle the size for example of the
σshape for the laplacian of gaussian? I have always compensated for that in the size of the filter… i.e.,
σshape = [1.0, 1.0, 0.5] - but I’m wondering if I should instead be leveraging ImageAxes to set the units, and then the σshape is simply
[1,1,1] ? I guess I’m confused on how the setting the units for the axes affects the sizes for the filters.
Thanks in advance for any guidance!